A tomography reconstruction of several serially sectioned slices using the slice and view facility of the FEI Helios 650 FIB SEM. The rectangular volume was sequentially sliced by the ion beam and the exposed cross sectional surface imaged in backscattered mode. Each section or slice was 20 nm apart and resulted in 246 images or slices. 3D reconstruction was done using the software packages ImageJ and Amira